Seminar Prof. Hyun-Woo Rhee [Seoul National University, Department of Chemistry] - Molecular Spatiomics by Proximity Labeling
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Date : 2024/05/09 5PM
Venue : Science Hall, B133
Abstract : Proximity labeling can be defined as an enzymatic "in-cell" chemical reaction that catalyzes the proximity-dependent modification of biomolecules in live cells. As this labeling reaction is proximity-dependent due to the short lifetime of reactive species, it can be used to map spatial proteomes, transcriptomes, and cellular networks. In our lab, we have developed a super-resolution proximity labeling technique (SR-PL) and we used it for architecture mapping of metabolic components of mitochondria in live mammalian cells and in mammalian tissues. Recently, we recognized parallels between spray-type modifications (e.g., Acetyl Spray, ADPR Spray, SUMO Spray, etc) and proximity labeling techniques, as both involve chemical interactions between electrophilic groups and nucleophilic moieties in close proximity. In this talk, I will explore how spray-type modifications can impact spatial biological components, offering a promising avenue for unraveling the complexities of spatial biology.