Seminar Prof. Myeong Hee Moon [Yonsei Univ.] - Field-Flow Fractionation and Mass Spectrometry: A Personal Retrospective
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Date: 2025/12/12 (FRI) 5pm
Venue: Science Hall B133
Abstract:
This lecture offers a reflective overview of my scientific odyssey in advancing Field-FlowFractionation (FFF) and Mass Spectrometry (MS) for native-state analysis of biologicalmacromolecules. Over the past three decades, my work on the gentle, open-channelprinciples of Flow FFF naturally extended into studies of biological particles such aslipoproteins and extracellular vesicles, which ultimately led me to a deeper engagement withmass spectrometry. Along this path, unexpected experimental challenges inspired newchannel designs, including the frit-inlet asymmetrical system and a hollow-fiber disposableplatform, as well as two-dimensional strategies combining isoelectric focusing with FFF. Coupling Flow FFF with MS, both off-line and in emerging miniaturized on-line formats,enabled size-resolved proteomic and lipidomic characterization relevant toneurodegeneration, cancer, and clinical biomarker discovery. This retrospective highlightsnot only the technical advances but also the curiosity, persistence, and meaningful turningpoints that shaped a lifelong pursuit of understanding complex biomolecular assemblies.